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Proteintech
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Proteintech
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Santa Cruz Biotechnology
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PeproTech
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Illumina Inc
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Journal: GeroScience
Article Title: Tilorone attenuates high-fat diet-induced hepatic steatosis by enhancing BMP9-Smad1/5/8 signaling.
doi: 10.1007/s11357-025-01685-8
Figure Lengend Snippet: Fig. 8 Summary of the results. After the 10 weeks tilorone treatment, the HFD-induced increases in complex II-linked oxidative phosphorylation and com- plex IV activity reduced and the HFD-induced decreases in BMP9, pSmad1/5/8, and PPARγ levels were normal- ized. PET/MRI showed increased tissue uptake of 18FDG. As a consequence, lipid content of the liver decreased, the glycogen content increased, and blood glucose level, body mass, and liver and adipose tissue weights were normal- ized. HFD, high-fat diet; BMP, bone morphogenetic protein; PPARγ, peroxi- some proliferator-activated receptor gamma; 18FDG, 18F-fluoro-2-deoxyglucose
Article Snippet: Then, the membranes were blocked in Tris buffered saline containing 5% skimmed milk and 0.1% Tween-20 (Sigma-Aldrich) for 1 h at room temperature and were incubated at 4 °C overnight with the following rabbit polyclonal primary antibodies: phospho-Smad1/5/8 (Smad1 [Ser463/465]/Smad5 [Ser463/465]/Smad9 [Ser465/467]; #AB-3848-J; Sigma-Aldrich); Smad1/5/8 (#56,656; Novus Biologicals; Centennial, CO, USA); PPARγ (#2435; Cell Signaling Technology; Danvers; MA; USA); PGC-1α (#2178; Cell Signaling Technology); TOM20 (#42,406; Cell Signaling Technology); BMP6 (#55,421–1-AP; Proteintech; Rosemont; IL, USA);
Techniques: Phospho-proteomics, Activity Assay